Silent superbug killers in a river near you how factory farms contaminate public water courses on three continents

Water downstream from factory farms harbours an invisible threat to people's health which could eclipse the COVID-19 crisis. The threat? Antibiotic Resistance Genes (ARGs) which are driving antimicrobial resistance the world's superbug crisis - projected to kill up to 10 million people annually by 2050. This publication reports the presence of ARGs in animal waste discharged from industrial farms into public waterways or onto soil (or crops) in four countries. Gauge community impact and sentiment regarding the issue was also highlighted. The water and sediment from public water courses connected to effluent discharges from 6-10 pig farms were tested in each of four countries (Canada, Spain, Thailand and the USA).

Areas within the United States at the Highest Risk for African Swine Fever, Classical Swine Fever, and Foot-and-Mouth Disease Introduction

Domestic livestock production is a major component of the agricultural sector, contributing to food security and human health and nutrition and serving as the economic livelihood for millions worldwide. The impact of disease on global systems and processes cannot be understated, as illustrated by the effects of the COVID-19 global pandemic through economic and social system shocks and food system disruptions. This study outlines a method to identify the most likely sites of introduction into the United States for three of the most concerning foreign animal diseases: African swine fever (ASF), classical swine fever (CSF), and foot-and-mouth disease (FMD). We first created an index measuring the amount of potentially contaminated meat products entering the regions of interest using the most recently available Agricultural Quarantine Inspection Monitoring (AQIM) air passenger inspection dataset, the AQIM USPS/foreign mail, and the targeted USPS/foreign mail interception datasets. The risk of introduction of a given virus was then estimated using this index, as well as the density of operations of the livestock species and the likelihood of infected material contaminating the local herds. Using the most recently available version of the datasets, the most likely places of introduction for ASF and CSF were identified to be in central Florida, while FMD was estimated to have been most likely introduced to swine in western California and to cattle in northeastern Texas. The method illustrated in this study is important as it may provide insights on risk and can be used to guide surveillance activities and optimize the use of limited resources to combat the establishment of these diseases in the U.S.

Reaction of the cereal market to the disruption of trade flows in Romania

The paper presents the reaction of the Romanian cereal market to the disruption of trade flows caused by certain shocks, such as the COVID-19 pandemic, which lead to changes with high impact on the functioning of this market, representing an important test for the resilience of the sector. Due to trade liberalization in global markets, including agri-food markets, the competitiveness of exports has become increasingly important, contributing to the creation of the country's competitive advantage. Any restrictions to trade in agri-food products can distort trade flows, and this disruption will have an impact on supply and prices. Maintaining a balance between imports and exports is essential to ensure domestic market stability. International trade in agri-food products plays an important role in global food security. The results show that Romania mainly exports unprocessed agricultural products, with cereals having the largest share in the export structure, cereal supply is dependent on climate change, yet it is one of the products with the lowest volatility. The cereal market shows a more elastic reaction to price responses, even though demand for staple foods is generally inelastic.

Face masks as sources of cross-contamination during food preparation

Amid the COVID-19 pandemic, mask-wearing has become a common practice in the foodservice industry to prevent the spread of respiratory diseases. Like kitchen utensils, a mask may serve as a vehicle for cross-contamination of pathogens during food handling. The objective of this study was to quantify cross-contamination between tasks of handling contaminated chicken and chopping lettuce. Chicken breasts were inoculated with a high or a low level of nonpathogenic Escherichia coli surrogates (ca. 6 or 4 log CFU/ml) and sliced for 1, 5, or 10 min. During slicing, duplicate, single-use medical masks were touched each minute. One mask was immediately sampled, but the second mask was used to contaminate lettuce by touching the mask each minute while chopping the lettuce for 5 min. E. coli were enumerated from the second mask and lettuce. Masks touched while slicing both high- and low-inoculated chicken showed significant contamination (0.8-4.9 log CFU/cm2) after each slicing scenario of 1, 5, or 10 min (P > 0.05). Lettuce was significantly contaminated regardless of inoculation level (1.0-3.2 log CFU/g). Slicing time was a significant factor in some cases (P < 0.05), whereas inoculation level was not (P > 0.05). Data indicate masks can be a source of cross-contamination if not replaced appropriately.

Resilience of U.S. cattle and beef sectors: lessons from COVID-19. (Special Issue: COVID-19 impacts on selected commodities.)

The upheaval wrought on the U.S. beef industry by the global COVID-19 pandemic carried with it several lessons that might help improve resiliency should there be a reoccurrence. First, the futures market for fed cattle fell well before cash prices, which sent a signal to market cattle early, and those who did so benefited. Second, the decline in futures anticipated the closure of slaughter plants and provided an opportunity to purchase and store beef primals in anticipation of future scarcity. Third, the beef industry has ways of slowing or stopping the pipeline of animals destined for feed yards and can "store" these animals in background feeding facilities or on pasture or rangeland. Producers who waited to sell feeder cattle benefited from higher feeder cattle prices once the processing facilities reopened. Fourth, cow slaughter plants responded to the pandemic and subsequent scarcity of labor much better than large fed-cattle plants. Cow plants are not as sophisticated and complex as fed-cattle plants. This relative simplicity may help explain the superior performance of these plants during the crisis. Sixth, the academic work on the value of building smaller plants as a response against concentration provides mixed results-these plants require more labor per animal and can be even more susceptible to labor scarcity. Seventh, the observed increase in boxed beef prices, even as fed cattle prices fell, demonstrates the risk-mitigating impact of producer ownership of downstream activities in the value chain.

The meatpacking industry's corporate exceptionalism: racialized logics of food chain worker disposability during the COVID-19 crisis

Building on theories of biopower and necropolitics, we detail how the meatpacking industry expanded corporate exceptionalism amidst the U.S. COVID-19 pandemic. Our analysis argues that the industry utilized three strategies to assert exceptionalism and secure increased production and profitability despite significant risks for meatpacking workers. First, the industry constructed COVID-19 as an urgent threat to the nation's meat supply, casting themselves as a critical economic linchpin. Second, the industry aligned themselves with heroic portrayals of meatpacking workers, deflecting criticism of their handling of the crisis. Third, the industry promoted images of themselves as competent stewards, meriting unfettered autonomy to manage workers' health risks. Detailing these strategies sheds light on how corporate exceptionalism functions within late capitalist food systems to further racialized logics of worker disposability. [ FROM AUTHOR] Copyright of Food, Culture & Society is the property of Routledge and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Negative Integration Is What States Make of It? Tackling Labour Exploitation in the German Meat Sector

In this article, we trace Germany's recent reform (and its precursors) seeking to remedy precarious working conditions in the meat sector. Focusing on an extreme case of labour exploitation, and asking how unique it is, allows us to uncover which institutional features of EU Member States condition the liberalization effects of negative integration. We thereby contribute to the literature on Europeanization, which has mainly emphasized weak industrial relations to account for the German meat industry's reliance on cheap migrant labour. Complicated enforcement structures, demanding requirements of administrative cooperation, and the complexities of an evolving case law, we argue, further contributed to the precarious conditions of migrant workers in Germany. Major COVID outbreaks in slaughterhouses created the political momentum for reform which specifically addresses this administrative side of labour protection, but remains limited to the meat sector – despite similar patterns of labour exploitation elsewhere.

Game meat procurement market in Poland in 2009-2021

Procurement of game animals is a major source of revenue for hunting clubs in Poland. For several years, the game meat buying market has been showing an upward trend, but this situation is also influenced by random factors that negatively affect the value of the game meat buying market. For several years in our country we have been struggling with the ASF virus, and since 2020, negative effects in the economy related to the occurrence of the SARS-CoV virus have been observed, also affecting the hunting sector with its activities. The aim of the study was to analyze the dynamics of game meat procurement in Poland in the years 2009-2021. The data concerned the three most important species, namely deer, roe deer and wild boar. The analysis covered the quantity of game meat, procurement value and the average price of game meat depending on animal species. The conducted research confirmed an upward trend in the volume and value of game meat procurement for all the analysed game species. Similarly, the average procurement prices of roe dee and wild boar meat with the exception of red deer, showed an upward trend. The study confirmed the negative impact of the ASF virus and the SARS-CoV-2 virus on the game meat buying market in Poland.

Wild meat consumption in zoonotic pandemics in Ghana

This study assessed wild meat consumption after the outbreak of COVID-19 in Ghana using the Health Belief Model framework. Data were collected through a household survey near two important wildlife-protected areas in Ghana. There was less wild meat consumption after the outbreak of COVID-19 than before the outbreak. About half of the respondents (51%) perceived themselves to be susceptible to diseases with proximal wildlife origin. The best model of a stepwise-multiple regression explained 49% of the variance, and three constructs (Perceived Susceptibility, Self-efficacy, and Perceived Seriousness) of the Health Belief Model were significant predictors of wild meat consumption weariness. Behaviour change interventions, based on creating awareness of the health risks of wild meat gathering and consumption, could be helpful in both public health and conservation circles. [ FROM AUTHOR] Copyright of Human Dimensions of Wildlife is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Special Issue: COVID-19 impacts on selected commodities. (Special Issue: COVID-19 impacts on selected commodities.)

This special issue consists of seven articles examining the impacts of the COVID-19 pandemic on the markets of selected commodities, including: seafood, lumber, crawfish, cattle/beef, fruits and vegetables in the USA, cotton and garments in Bangladesh, and fuel oil globally.

Understanding small- and very-small-scale meat processors in Missouri to strengthen the local supply chain

Promoting local food systems is crucial to provid-ing a more viable economy, eco-friendly produc-tion, and equal opportunities for producers, con -sumers, and communities. Meat processors are critical to local meat producers and the meat supply chain. However, various barriers have restricted small-scale meat processors and challenged the lo -cal meat supply chain. Although local food systems have gained enormous scholarly attention, little at-tention has been devoted to specifically exploring the meat processing sector. This study investigated the characteristics and challenges of small-scale (<750 employees) and very-small-scale (<200 em-ployees) meat processors in Missouri. Twenty-six meat processors participated in an online survey through Qualtrics, a mail survey, or a structured phone interview between May 2021 and March 2022. We identified the characteristics and con-straints related to their businesses. The analysis re-vealed that 76% of meat processors perceived that their business was in better or much better condi-tion than before the COVID-19 pandemic, reflect-ing their adaptability to the disrupted meat supply chain. However, small-scale meat processing facili-ties were limited by the labor shortage, complicated regulations and high regulatory compliance costs, a lack of consistent supply, and limited access to tools and equipment. More integrated work is needed to aid smaller processors in positively im-pacting the local community and environment through locally sourced meat production. This study contains helpful implications for state-level policymaking, extension programs, and future re-search directions.

Webinars applauded by the chicken meat industry during the pandemic

The AgriFutures Chicken Meat RD&E program funded Training and extension for the chicken meat industry project quickly pivoted to delivering online webinars in response to the global human health pandemic in 2020 caused by the SARS-CoV-2 virus (COVID-19). The change to an online format was met with enthusiasm by all sectors of the industry. Between July 2020 and April 2022, 14 webinars have attracted 927 participants from 1622 registrationsan average attendance rate of 57%. Key topics such as litter management, environmental sustainability, nutrition, health, biosecurity and bird behaviour have been discussed with 27 researchers, encompassing 34 individual research projects. The positive feedback from industry participants in an independent mid-term project review, where 99% are interested in attending more webinars, coupled with high attendance and registration rates, indicate that this delivery format has an important role to play alongside face-to-face activities into the future.

Vulnerability and coping strategies within wild meat trade networks during the COVID-19 pandemic.

Measures adopted to prevent the spread of COVID-19 and economic shocks caused by the pandemic have affected food networks globally, including wild meat trade networks that support the livelihoods and food security of millions of people around the world. In this article, we examine how COVID-related shocks have affected the vulnerability and coping strategies of different actors along wild meat trade networks. Informed by 1,876 questionnaires carried out with wild meat hunters, traders, vendors, and consumers in Cameroon, Colombia, Democratic Republic of Congo (DRC), and Guyana, the article presents qualitative evidence as to how COVID-19 impacted different segments of society involved in wild meat trade networks. Our findings largely align with McNamara et al. (2020) and Kamogne Tagne et al.'s (2022) causal model hypothesising how the impacts of the pandemic could lead to a change in local incentives for wild meat hunting in sub-Saharan African countries. Like McNamara et al. (2020) and Kamogne Tagne et al. (2022), we find that the pandemic reduced wild meat availability for wild meat actors in urban areas while increasing reliance on wild meat for subsistence purposes in rural areas. However, we find some impact pathways to be more relevant than others, and also incorporate additional impact pathways into the existing causal model. Based on our findings, we argue that wild meat serves as an important safety net in response to shocks for some actors in wild meat trade networks. We conclude by advocating for policies and development interventions that seek to improve the safety and sustainability of wild meat trade networks and protect access to wild meat as an environmental coping strategy during times of crisis.

Spatial Dimensions of Vulnerability: Meatpacking, Socioeconomics, and the Outbreak of COVID-19 in the High Plains of Texas

In May 2020, 2 months after COVID-19 arrived in the High Plains of Texas, meatpacking plant workers were discovered to be contracting the virus in large numbers. Working conditions in the plants-close spacing on the disassembly lines, cold temperatures, noise (shouting to be heard), etc.;along with congregant settings among the immigrant workers before and after work-were all implicated in the infections. Although much has been written on the vulnerabilities of meatpacking workers, little research has investigated the spatial spread of the virus. In this study we analyze COVID-19 case rates for May 15 (the first spike in daily case numbers), for the 41 counties of the region in relation to meat-packing influence, ethnicity, and socioeconomic structure of the counties. We find that meatpacking influence had the strongest relationship to COVID-19 rates across the counties;that the presence of Asian and African immigrants was also significant;and that rurality and isolation insulated more than half the counties from high virus rates. Further analysis, for later spikes in cases (July 1 and November 25), revealed a decline in meatpacking influence, a surge in COVID-19‘s infection of counties with large domestic minorities, and an amplification of low COVID-19 cases for rural, older, Anglo counties. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.

Glycyrrhizin inhibits swine acute diarrhea syndrome coronavirus infection via the HMGB1/TLR4/JNK signal pathway

Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly discovered enteric coronavirus, is the etiological agent that causes severe clinical diarrhea and intestinal pathological damage in piglets. In this study, Vero E6 and IPI-2I cells were pretreated with different concentrations of glycyrrhizin (GLY) for 2 hours, and then infected with different concentrations of SADSCoV, aiming to investigate the inhibitory effect of GLY on SADS-CoV. Western blot and TCID50 results revealed a significantly decreased N protein expression and viral titer, indicating that GLY can inhibit the infection of SADS-CoV. Vero E6 and IPI-2I cells were pretreated with different concentrations of GLY for 2 hours and infected with SADS-CoV. Western blot results showed that when the concentration of GLY was 0.8 mmol/L, the expression of N protein decreased significantly, indicating that GLY inhibited the invasion of the virus. At first, cells were treated with 0.4 mmol/L GLY, and cell samples were collected at 2 hours, 6 hours and 12 hours after being infected with SADS-CoV for analysis, and the expression of N protein were found to be significantly reduced at all points, indicating that GLY had a significant inhibitory effect on the replication of the virus. GLY is a competitive inhibitor of high mobility group box 1 (HMGB1), and the receptors of HMGB1 mainly include TLR4 and RAGE. Based on this fact, the mutant plasmid at the key sites of HMGB1 (C45S, C106S, C45/106S) and the siRNA of the RAGE receptor were transfected to Vero E6 cells and infected with SADS-CoV, and the cell supernatant and samples were harvested. The western blot and TCID50 results showed that the expression of N protein and the virus titer were decreased, suggesting that GLY exerts its function by affecting the binding of HMGB1/TLR4/RAGE during SADS-CoV infection. To further explore the signaling pathway through which GLY functions, Vero E6 and IPI-2I cells were inoculated with SADS-CoV, and cell samples were harvested, western blot was used to detect the changes of MAPK proteins. The results showed that the protein expression levels of p-p38, p-JNK and p-ERK were up-regulated in the early and late stages, indicating that the MAPK pathway was activated by SADS-CoV infection. Vero E6 and IPI-2I were pretreated with different concentrations of GLY and TLR4 inhibitor TAK for 2 hours and infected with SADS-CoV. Protein samples were harvested and analysed by western blot which showed a decreased p-JNK and N proteins, while other proteins showed no significant changes. These results indicated that GLY and TAK regulated the phosphorylation of JNK but did not regulate the phosphorylation of p38 and ERK. Also, Vero E6 cells were treated with HMGB1 antibody, the siRNA of HMGB1 and HMGB1 mutants plasmid, and infected with SADS-CoV. Protein samples were harvested, western blot results showed that phosphorylation of JNK decreased, indicating that HMGB1 affected JNK phosphorylation. Finally, Vero E6 and IPI-2I cells were pretreated with different concentrations of JNK inhibitor SP600125 to infect SADS-CoV, western blot, TCID50 and IFA results showed that the expression of N protein and virus titer, as well as virus replication were reduced, indicating that SP600125 inhibited virus replication. In conclusion, our results revealed that GLY can inhibit in vitro replication of SADS- CoV, mainly through the HMGB1/TLR4/JNK signaling pathway. The discovery of this pathway provides theoretical support for the research of novel anti-SADS-CoV drugs.

Assessing disease risk perceptions of wild meat in savanna borderland settlements in Kenya and Tanzania

Wild meat hunting and trade across African savannas is widespread. We interviewed 299 people in rural settlements along the Kenya-Tanzania border to examine impacts of COVID-19 on wild meat consumption and perceptions about wild meat activities associated with zoonotic disease risks. Education level played a key part in understanding COVID-19 transmission. Information about the pandemic was mostly acquired from the media. Nearly all respondents recognized that COVID-19 originated in China. As many as 70% reported no impact of COVID-19 on wild meat consumption;some believed that there was an increase. Over half of the respondents believed that consumption of wild meat leads to food-borne illnesses. Respondents recognized disease risks such as anthrax and brucellosis and accepted that people slaughtering and handling wild meat with open cuts were at greater risk. Ungulates were the most consumed animals, followed by birds, rodents, and shrews. Respondents perceived that hyenas, monkeys, donkeys, and snakes were riskier to eat. More than 90% of the respondents understood that handwashing with soap reduces risks of disease transmission. Country level (11 answers), education and gender (three answers each) and household economy (158 answers) were significant. Country differences were linked to differences in nature legislation;50% of Kenyan respondents believed that wild meat should not be sold because of conservation concerns. Men were more worried about getting COVID-19 from live animals and perceived that wildlife should not be sold because of conservation reasons. Overall, there was a very strong inclination to stop buying wild meat if other meats were less expensive. Our results allow us to better understand the impact of the COVID-19 pandemic on wild meat-related activities. Differences between countries can frame the attitudes to wild meat since wild meat trade and consumption were found to be country specific.

Meta-Analysis of COVID-19 Spread in Meat Processing Plants and Recommended Practical Actions

Recently, the spread of the coronavirus disease 2019 (COVID-19) has increased among workers of meat processing plants (MPPs) around the world. This study reviewed the possible routes of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and useful actions against it in slaughterhouses. The results revealed that the main factors for the spread of the virus included low indoor temperature, crowded area, wrong standing along production lines, contamination of high-touch surfaces, difficult education of workers with diverse native languages, low financial income, large MPPs with over 10 million Ib of packed meat per month, higher speed of production lines with 175 birds/minute, temporary contract of the workers, and weak approach of some meat processing companies against COVID-19 infection such as National Beef. COVID-19 transmission rate was 24 times higher among the workers of MPPs than among the population of the US. The practical actions against the spread of the virus were mainly marker using for remembering the previous location, mandatory mask use, especially FFP2/3 masks, and decentralization of large MPPs. By using the results of this study, slaughterhouse managers would be able to significantly control the spread of SARS-CoV-2 and future bio-threats to workers of MPPs and even to society. © 2022 The Author(s);.

Our ‘Pacific family'. Heroes, guests, workers or a precariat?

Australia launched a Seasonal Worker Programme (SWP) in 2012, shortly after a similar scheme in New Zealand, to bring seasonal workers from Pacific Island Countries (PICS) to work in agriculture. The scheme was seen as a potential ‘Triple Win' with sending and receiving countries, and workers' households, benefiting. Workers' remittances contributed to welfare, especially housing and education, and small business establishment, but there were social costs associated with repeated absences. In 2018, Australia introduced the Pacific Labour Scheme (PLS) to extend guestwork opportunities into other areas of non-seasonal labour shortage such as aged care, tourism and meat processing. The shortage of local labour during COVID-19 demonstrated that Pacific guestworkers were invaluable to Australia, and in 2022 the schemes were revamped and expanded further as the PALM (Pacific Australia Labour Mobility) Scheme. Concern over a Chinese threat in the region gave further support for the expansion. PICs expressed concerns about exploitative practices, while higher rates of participation increased the potential for an incipient brain drain from the PICs, with wages roughly four times those at home, as migrants now left non-agricultural jobs. The expanded scheme continues to favour Australian employers leaving questions over, equity, uneven development and the future of the PICs.

Interaction between target peptide and complementary peptide by bimolecular fluorescence complementary technology

[Objective] Using the bimolecular fluorescence complementation (BiFC) technology, the present experiment aimed to study the interaction relationship and localization of the target peptide and the complementary peptide based on the porcine epidemic diarrhea virus (PEDV) S protein receptor binding site peptide in living cells, so as to provide the foundation and theoretical support for the further use of the peptide in the detection of porcine epidemic diarrhea virus. [Method] The target peptide was designed according to the physical and chemical characteristics of the target protein, such as the amino acid composition, the type of charge, the ability to form intennolecular hydrogen bonds, the strength of polarity, and hydrophobicity;According to the amino acid composition of the target protein, a complementary peptide that interacted with it in theory was designed, and the target peptide and complementary peptide were predicted and analyzed by using bioinfonnatics tools;The target peptide and complementary peptide were inserted into the pBiFC-VC155 and pBiFC-VN173 vector, which was double digested by the EcoRI/XhoI and NotI/SalI, respectively, verified by enzyme digestion and sequencing, and then transfected into Vero cells to study the interaction between the target peptide and the complementary peptide, and the precise localization of BiFC complex in cells. [Result] Bioinfonnatics analysis showed that the target peptide and complementary peptide had hydrophilic and hydrophobic domains, respectively, and the hydrophilic domains were both positively and negatively charged, which could generate electrostatic attraction. The results of enzyme digestion and sequencing showed that the pBiFC-VC155-target peptide and pBiFC-VNI73-complementary peptide plasmids were successfully constructed;Cell transfection experiments showed that the target peptide and complementary peptide could form BiFC complexes in Vcro cells after co-transfection of recombinant plasmids, indicating that they could interact with each other;Indirect immuttolluorescence assay confirmed that the BiFC complex was mainly distributed in the nucleus. [Conclusion] It was confirmed that the peptide designed based on the PEW/ S protein receptor binding site can interact with each other in living cells, demonstrating the feasibility of the peptide for detection.